Anisotropic PLGA microsphere/PVA hydrogel composite with aligned macroporous structures for directed cell adhesion and proliferation

The fabrication of bio tissues using anisotropic constructs capable repairing and/or replacing diseased remains a significant challenge in tissue engineering. Therefore, this study investigated the an PLGA-microspheres/PVA-hydrogel composite novel directional freezing-thawing (DFT) technique. DFT technique altered properties composite, such that its compressive strength improved from 14.64 ± 1.09 MPa after three cycles to 45.77 6.73 nine cycles. utilization was also shown enhance proliferation and adhesion cultured chondrocyte cells. obtained results demonstrated constituted facile method fabricate microsphere/hydrogel composites with directed cell characteristics.